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Largest changes:

Moved from 6v6 to 5v5 (1 tank 2 dps 2 support)
Reworked respawning system, base respawn time reduced from 10 to 8 seconds, consecutive deaths now slightly affect respawn times to decrease snowballs
Passive ultimate charge generation increased by 20%

Role Passives

Supports now regenerate 10 health per second after not taking damage for 2 seconds

Tanks now have damage reduction based on the amount of enemies shooting at them (10% per additional target)

Mercy Rework

Resurrect

Moved back to Ultimate
Remains single target, no longer has a cast time
Once completed, you will have a 15 second window to perform another Resurrect
Each use resets the cooldown of Guardian Angel
Targets are revived at half health, and recover the rest over 10 seconds

Torchbearer: New E ability

20 second cooldown
Cooldown recharges twice as fast for 10 seconds after using Resurrect, as long as the resurrected target is alive
Upon activation, heals all allies within 15 meters by half of their missing health, and cleanses them of stuns
Enemies within LoS will have their damage reduced by 50% for 2.25 seconds
Briefly allows usage of Guardian Angel with no target

Bastion Rework (based on OW2)

Configuration: Sentry

No longer prevents movement
Drains 20 ammo per second while in use (in addition to ammo usage), ends when running out of ammo (cannot reload)
Damage decreased by 30%
Now has a 10s cooldown
Damage reduction replaced with 100 armor
Transforming back into Recon is now instant

Configuration: Artillery

Replaces Configuration: Tank
Shoot 3 spots on the map from an overhead view to drop artillery strikes that deal 300 damage in a 6 meter radius

Hanzo

Storm Bow

Headshot damage reduced from 250 to 180. now grants 2 Storm Arrows

Lunge

Cooldown reduced from 5 to 3s

Storm Arrows

Changed from a single cooldown of 5 arrows to a charge system of 8 arrows, recharging 1 every 2 seconds.
Now requires a continuous button hold (Moved from Ability 2 to Secondary Fire)
Damage reduced from 70 to 49

this guy

Health reduced from 225 to 200

Combat Roll

Cooldown now decreases by 0.5 seconds per headshot
Distance increased by 20% while on the ground

Flashbang

Now is a root (prevents movement but not shooting or non-movement abilities, still interrupts most abilities)

Deadeye

Now grants 100 temorary health

Sigma

Experimental Barrier

Instead of a recharging barrier, it's now a cooldown ability, with a max duration of 1.75 seconds after being placed; cooldown is 7 seconds

New Passive: Hold jump to reduce your gravity to 0, slightly increase projectile and move speed (on a resource meter)

Kinetic Grasp

Cooldown decreased from 12 to 10s

Accretion

Long range directs now decrease the cooldown by 3 seconds and make Sigma say his Yes voice line (YEP rock)

Ana

Sleep Dart

Cooldown reduced from 12 to 10s
Cooldown now starts when the target wakes up (only if you land a close range sleep)
Added icon on slept targets that your whole team can see through walls